At The Ex-Vivo Research Centre (EVRC) we provide a novel alternative to large animal experimentation via ex-vivo organ and tissue perfusion. We evaluate materials, medical devices and therapies in physiological environments where function and metabolism of tissues are restored, or pathophysiological environments where injury and disease are induced. The development of devices and therapies follows an established roadmap, from in vitro cultures, through small animal models into large animal models before evaluation in humans. This roadmap often limits the development of potentially life-saving innovations. A major bottleneck occurs between the small animal and large animal stage, where experiments are ethically challenging and prohibitively expensive. We provide a cost effective, higher throughput and ethical alternative to large animals to lower the risk of evaluating potential innovations in a physiological system. We have developed a wide range of ex-vivo organ and tissue perfusion protocols using material sourced from the abattoir setting. This has multiple benefits; Highly cost effective - Reducing experimental costs by 93-95% Ethical - Tissues sourced from abattoir pigs Higher throughput - Ability to perform multiple experiments per day Better translation - Physiological environments We operate as a community interest company (CIC), so we do not have shareholders or dividends. Our profit is used for specific purposes (R&D activities that benefit patients, developing alternatives to animal research, finding environmentally improved solutions to healthcare research, providing careers to individuals affected by traumatic limb loss, and supporting individuals with the ability but not the opportunity to develop a career in biomedical research).