PPBI - Portuguese Platform of Bioimaging is a National Research Infrastructure for Open-access to Bioimaging resources and is a biological Node of EuroBioimaging ERIC, PPBI Euro-BioImaging Node Organized as a consortium of top research universities and institutes in Portugal, the PPBI services focus on advanced microscopy and processing/analysis of images in the life-sciences, from cell & developmental biology, neurosciences, oncobiology, immunology, infection, and regenerative medicine. Currently, PPBI consists of 16 Nodes distributed by 3 regional poles (DOURO & MINHO, MONDEGO & BEIRAS, TEJO & ALGARVE), comprising more than 100 high-end equipment resources supported by Ph.D. experts in bioimaging. Access to PPBI resources and services is open to all scientific community, as well as industry. PPBI also wants to boost collaboration between researchers, institutions, and industry to strengthen both basic and applied research, matching high-impact R&D projects with state-of-art bioimaging resources and expertise. Through shared coordination, strategic investments, and efficient allocation of resources, PPBI wants to give access to cutting-edge technological resources to national researchers, create conditions to attract international researchers and integrate Portugal in the international BioImaging roadmap. PPBI nodes provide a wide variety of services in bioimaging, including access to imaging equipment, to wet lab and sample preparation equipment, cluster access for computation and data storage, image analysis and training (courses and workshops, online tools). This infrastructure also offers support of animal facilities (model and non-model organisms) and equipment operation, consulting and project planning, technology development, and outreach activities. PPBI integrates the Portuguese Roadmap for Research Infrastructures of Strategic Interest