The HPLC symposium series is known as the world leading conference on liquid phase separations and related technologies. Its program covers all aspects of separation sciences in liquid and supercritical fluid phases as well as hyphenation with advanced detection technologies in particular mass spectrometry. The program will span from fundamentals and theory of chromatographic separations and detection principles, over methodological and technological advances including separation materials, column technologies and instruments, to applications in various fields and quality assurance aspects. The symposium will feature workshops and tutorials, plenary and keynote lectures from the leading scientists in the field. HPLC 2021 will have a big exhibition and vendor seminars in which attendees can see the latest innovations from the leading vendors in the field. Duesseldorf is an important and interesting city at the heart of Europe. It ranks among the six foremost cities worldwide in terms of quality of life. Guests will be spoilt for choice with attractions such as the Altstadt – as the historic part of the city –, or its classy shopping mile – Koenigsallee –, the Rhine embankment promenade, which is perfect for biking and strolling, or the architecturally and gastronomically appealing MedienHafen (Media Harbour) with its quality museums, theatres, concerts, cabarets, the opera house, and annual event highlights. Mark your calendar! We look forward to your participation.