Unmet Need: There is an unmet need for a single-column, universal platform method to reliably purify tagless recombinant proteins. Affinity tag methods followed by tag removal, although commonly used, are unreliable, complex, lead to productivity losses in research, and are impractical for biopharmaceutical production. Solution: The key capabilities and advantages of the novel technology for protein purification provided by Protein Capture Science are: 1. Provides tagless purified proteins via tag self-cleavage during affinity purification 2. Gentle cleaving based on small pH change 3. Reproducible purity and recovery of target 4. Tunable target-independent cleavage rate 5. Scalable from laboratory to manufacturing 6. Simple and inexpensive buffer requirements 7. Opportunity to merge multi-platform processes into one platform (from R&D to production) Product: Intein-based product is a resin containing proprietary intein-based fragment that allows traceless and tagless self-cleaving protein purification. Two forms of product are available to purchase: 1. A kit containing pre-packed column (1mL or 5 mL) 2. A bulk resin (10 mL to L scales)