The North American 3Rs Collaborative (NA3RsC) is an non-profit organization dedicating to advancing science, innovation, and research animal welfare through collaborative 3Rs efforts. Work in the 3Rs space not only requires passion and dedication, but also collaboration and partnership. Such partnerships are essential for both the development of new 3Rs approaches and their implementation in practice. The objectives of the NA3RsC are to facilitate communication and sharing among those in North America working to bring transformational solutions that advance science and animal welfare and to provide opportunities for scientists and organizations across the life sciences sector, nationally and internationally, including universities, industry, other research funding sources and regulatory authorities to collaborate.