Established in 2011 by a group of postdoctoral researchers at Stanford University, Bio-protocol aims to improve research reproductivity by sharing and discussing life science research protocols. Bio-protocol journal is a peer-reviewed protocol e-journal publishing high-quality life science protocols, and it has been listed by PubMed Central and Web of Science. Bio-protocol also runs a sister database, called Bio-101, which is an open platform for sharing protocols as well as asking and answering questions. Bio-protocol has built tight collaborations with several high profile research journals/publishers including Science/AAAS and eLIFE to advance their mutual goal of making the scientific literature more efficient and reproducible.