We are a consortium of university-based microscopy facilities united by values of collaboration, accessibility, excellence and innovation. Each year, over 3,500 researchers from universities and industry use our instruments and expertise in facilities around Australia. Over 150,000 trainee microscopists around the world use our online training tools. Microscopy Australia is funded by federal (NCRIS) and state governments and participating institutions. We report high-impact science outcomes from this investment in our annual Research Highlights and online news. We also engage the public in science with unique exhibitions and learning resources. Microscopy Australia enables access to an array of high-end microscopy platforms and associated technical expertise in strategic locations to efficiently service Australia’s microscopy needs. Microscopy Australia also has formal connections with a range of other specialised linked laboratories. Mission: to empower Australian science and innovation by making advanced microscopes accessible to all researchers. Vision: to provide world-leading microscopes and expertise that keep Australia at the forefront of global research.