France-BioImaging is the National Infrastructure in Biology and Health in the field of biological imaging. Our primary mission is to develop and provide access to innovative instruments and imaging technologies to scientists, and our philosophy is to associate leading R&D research teams with service facilities. This unique coordinated infrastructure gathers 24 large biological imaging facilities and 34 laboratories specializing in R&D for imaging in 6 local Nodes and one transversal Node dedicated to bioimage informatics. FBI aims at creating the most efficient adoption of the latest advances in all technologies and methods related to microscopy, by the users of the imaging facilities. These technologies and methods, reinforced by a strong support in computational analysis, provide quantitative measures and integrative understanding of a wide range of cell and tissue activities in biological models, from the simplest, to small animals in normal and pathological situations. The tryptic “Innovation, Training, Access” is the backbone of our activities, which are to disseminate and invent new imaging technologies, participate to national and international educational and training programs, make them accessible to national and international, academic or private users. Two main categories of technologies are available within France BioImaging (>350 set-ups): • Light Microscopy Imaging Systems (including regular and automated wide field microscopy, Laser Scanning confocal microscopes, Spinning Disk confocal microscopes, light-sheet…) • Advanced and Innovative Imaging Systems are also accessible. They cover the following technologies and methods: High-resolution & single molecule tracking, Electron Microscopy & Multimodal Imaging, Confocal with innovative modalities, Fluorescence spectroscopy in microscopy, In depth & Intra-vital Imaging, high-throughput.